RUO Cell Sourcing for Humanized Mice: A Validation Checklist

Humanized mouse models, especially NSG mice, are invaluable for immunology, oncology, and regenerative medicine. But the success of these models depends on sourcing high-quality RUO CD34+ cells.

The Cost of Poor Sourcing

Low-quality or bead-labeled cells can produce inconsistent engraftment, wasting weeks of animal study time and thousands of dollars in costs. For labs investing in these studies, validation is non-negotiable.

The Validation Checklist

1. Source Transparency: Donor eligibility, cord vs. peripheral vs. mobilized blood, and handling conditions should be disclosed.
2. Isolation Method: Confirm whether the supplier uses column or column-free methods, and whether Ficoll was used.
3. Bead Status: Ask explicitly whether cells are bead-free or bead-labeled. Bead-free is preferred for early kinetics.
4. Viability and Potency: Post-thaw viability should exceed 90%, and functional CD34+ per vial should be disclosed.
5. Engraftment Validation: Look for early (2–4 week) kinetics, long-term persistence, and direct head-to-head comparisons.
6. Reproducibility: Ensure consistency across donor lots.

FerroBio: Raising the Standard

FerroBio delivers standardized, bead-free RUO CD34+ cells with proven superior engraftment performance. Each vial meets strict criteria for potency and reproducibility, giving researchers confidence in their animal model investments.